Recombigen Bilirubin Total & Direct is a dual reagent system used for the simultaneous measurement of both total bilirubin and direct bilirubin levels in serum or plasma samples. The assay employs the Diazo method in an end-point format, where bilirubin reacts with a diazo reagent to form a colored complex. The intensity of this color is directly proportional to the bilirubin concentration, which can be measured spectrophotometrically.
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